Lentinan-Based Multifunctional Composite Hydrogel for Wound Healing

Abstract

Conventional wound dressings suffer from unbalanced tissue adhesion, skin-mismatched mechanical properties, and insufficient long-term bioactivity. Herein, a dual physically cross-linked multifunctional tissue adhesive lentinan–polyvinyl alcohol (PVA)–tannic acid membrane (LPTM) was fabricated via a freeze–thaw-assisted immersion strategy. The optimized LPTM-3 hydrogel patch, with a stable network formed by PVA crystalline cross-linking and hydrogen bond cross-linking, achieved a balanced tensile strength of 16.1 MPa and an elongation at break of 149.3%, robust yet non-damaging porcine skin adhesion of 163.17 kPa, antibacterial activity, and good biocompatibility with a hemolysis rate below 2.24%. Notably, LPTM-3 promoted fibroblast migration and modulated inflammatory cytokine expression in vitro, and achieved 99.06% full-thickness wound closure in rats on day 14, matching a commercial dressing. This work provides a promising candidate for clinical wound repair and a facile design strategy for polysaccharide-based bioadhesive materials.