Characterization of Catechol-Type Siderophore(s) from P. mosselii CFML 90-83

Abstract

Iron is essential for many biological processes, where it is used as a cofactor. Even though it is abundant in the Earth’s crust, it is in an insoluble form unavailable to microorganisms in the soil. To overcome this, bacteria produce siderophores, which are secondary metabolites that have a high affinity for iron. This allows bacterial cells to obtain insoluble iron that is essential for survival. Pseudomonas mosselii, a Gram negative bacterium, was isolated from soil. It was selected for further analysis as it was found to produce siderophore(s) under iron-limited conditions, which have not been characterized previously. Therefore, we aimed to purify and structurally characterize the siderophore(s) produced by P. mosselii. Our research revealed a catechol-type siderophore with fluorescence, and preliminary structural analysis revealed the presence of two molecules: 2,3-dihydroxybenzoic acid and 2,3-dihydroxybenzoic acid + methyl serine.