Zhejiang Center for Disease Control and Prevention

Accurate β-Casein Allergen Quantification in Processed Foods via an Isotope-Labeled Full-Length Protein Internal Standard

Abstract Accurate quantification of cow’s milk allergens in processed foods by UPLC-MS/MS is challenged by enzymatic digestion variability and complex matrix effects. β-Casein, a major milk allergen, requires reliable quantification. Herein, we developed a novel method for quanti...

Genomic Characterization of Clinical Group B Streptococcus Isolates from Zhejiang, China: Serotypes, Clonal Complexes, and Predicted Antimicrobial Resistance and Virulence Gene Profiles

This study aimed to characterize the population structure, predicted antimicrobial resistance gene profiles, and virulence gene repertoire of Group B Streptococcus (GBS) in Zhejiang Province, China, using whole-genome sequencing (WGS). Forty GBS isolates from clinical specimens u...

Associations between vaccine misinformation and influenza vaccine uptake: a population-based interrupted time-series study in China

Abstract Vaccine misinformation is linked to vaccine hesitancy, yet real-world evidence of its association with vaccine uptake remains limited. Most existing studies rely on self-reported intentions or experimental designs, offering little insight into population-level behavioura...

Prevalence and geographic variation of enteric pathogens among asymptomatic individuals in China: a nationwide, cross-sectional, multiplex molecular survey

The increasing use of multiplex molecular assays has improved the detection of enteric pathogens, but baseline carriage in asymptomatic populations remains poorly characterized, complicating the interpretation of positive test results. We aimed to investigate the prevalence and g...

Construction of a drug screening model for Mycobacterium abscessus infections

Abstract The management of Mycobacterium abscessus infections is critically hindered by its inherent multidrug resistance and the lack of effective therapies, necessitating advanced preclinical models for drug discovery. Here, we developed a sequential screening platform, progres...

Efficacy of hemodynamic profiling-guided antihypertensive therapy in elderly patients with early morning hypertension: a randomized controlled multicenter clinical study

Abstract Objective To evaluate the efficacy of hemodynamic profiling (HDpro)-guided antihypertensive therapy in elderly patients with uncontrolled morning hypertension. Methods A multicenter randomized controlled trial was conducted in 9 hospitals in Zhejiang Province, enrolling...

Bacillus subtilis extracellular vesicles surface-displaying superoxide dismutase attenuate alcoholic liver injury by activating the Nrf2/HO-1 axis

Alcoholic liver disease (ALD) represents a globally prevalent progressive hepatic disorder with continuously rising incidence. Its pathological mechanisms mainly stem from the direct toxic effects of alcohol metabolites, accompanied by aggravated oxidative stress, lipid overaccum...

Spatiotemporal dynamics and cross-regional transmission network of HIV-1 CRF67_01B in six provinces in China

Cross-regional transmission of emerging recombinant strains poses a major challenge to HIV-1 prevention and control. HIV-1 CRF67_01B was first identified in China in 2013 and has since been reported in multiple provinces. This study aimed to elucidate the origin and cross-regiona...

Type VI secretion system genes sciG and sciS facilitate biofilm formation of Salmonella enterica serovar Typhi

Biofilm formation is a crucial survival strategy for Salmonella enterica serovar Typhi ( S. Typhi), enabling it to withstand adverse environmental conditions and host immune responses. This process is tightly regulated by complex genetic networks. The type VI secretion system (T6...

Motivational profiles and exercise in late adolescents: a cross-sectional study of autonomy and behavioral engagement

Abstract Objective Physical inactivity among adolescents is a worldwide public health concern. This study investigated the psychological motivation of adolescent exercise and the mechanisms by which diverse motives translate into actual behavioral engagement. We aimed to provide...

Potential Mechanisms Linking Excessive Testosterone to PMOS: Insights from Network Toxicology and Machine Learning

Background/Objectives: Polyendocrine metabolic ovarian syndrome (PMOS) is characterized by hyperandrogenism, particularly excessive testosterone, as a core clinical feature and a key pathogenic metabolite, yet its molecular mechanisms remain incompletely understood. Methods: This...

Gut microbiota dysbiosis correlates with reduced drug exposure in patients with multidrug-resistant tuberculosis

Although systemic exposure to anti-tuberculosis agents is critical for therapeutic efficacy, substantial inter-individual variability of drug exposure persists despite standardized dosing. Emerging studies suggest that the gut microbiota may influence drug metabolism and host pha...

Type I and II pili mediate transcytosis of Streptococcus pneumoniae across blood-brain barrier cells in the pathogenesis of pneumococcal meningitis

Streptococcus pneumoniae , a common pathogen to cause pneumonia and otitis media, is also the most important causative agent of bacterial meningitis in humans. Previous studies have shown that S. pneumoniae strains can be pilus-negative, type I pilus- and/or type II pilus-positiv...

Impact of the evolution of national ART guidelines and Initiation criteria on low–level viremia trends and predictors in Anhui Province: a sequential period analysis

Over the past two decades, China’s National Free Antiretroviral Treatment Program has undergone significant paradigm shifts. However, how evolving national ART guidelines and initiation criteria have shaped the long-term incidence of persistent low-level viremia (LLV) remains poo...

A Label‐Free Bioluminescent scFv‐NLuc Fusion Probe Enabling a Smartphone‐Based Sensitive Immunoassay Platform Using Lomefloxacin as a Model Analyte

ABSTRACT Enzymes as labeling agents enhance lateral flow immunoassays (LFIA). However, their biological instability and reliance on labor‐intensive chemical conjugation hinder practical high‐throughput screening of food contaminants. To address this, we report a rapid, label‐free...

Insulin resistance as a potential mediator between central obesity and diabetic kidney disease risk in type 2 diabetes

Abstract Obesity is a key risk factor for diabetic kidney disease (DKD). However, BMI fails to capture body fat distribution. We hypothesized that central obesity, rather than generalized obesity, is associated with DKD risk in patients with type 2 diabetes mellitus (T2DM), and t...

ACE2-PNA conjugates exploit viral endocytosis for targeted intracellular delivery and exhibit dual antiviral efficacy against SARS-CoV-2

The COVID-19 pandemic and its protracted consequences underscore the urgent need for more effective antiviral strategies. Current antiviral strategies face a persistent challenge achieving sufficient viral suppression while minimizing off-target toxicity, particularly against suc...