West African Centre for Cell Biology of Infectious Pathogens

Molecular and functional analysis of calcium binding by a cancer-linked calreticulin mutant

Abstract Calreticulin (CRT) is an endoplasmic reticulum (ER) chaperone with low affinity calcium binding sites in its C-terminal domain. This region is altered by somatic mutations in the CRT gene ( CALR ), which drive a subset of myeloproliferative neoplasms (MPN). Perturbations...

Development and comparative evaluation of real-time PCR and colorimetric loop-mediated isothermal amplification (LAMP) assays for the detection of infectious spleen and kidney necrosis virus (ISKNV) in tilapia

Infectious Spleen and Kidney Necrosis Virus (ISKNV) is a highly lethal pathogen posing a major threat to global aquaculture. The recent emergence of ISKNV on the African continent has had devastating impacts on the tilapia fish industry. There is a critical need for rapid, sensit...