UCL Biomedical Research Centre

Protocol for genome-wide differential methylation using long-read DNA sequencing in the cortex of a mouse model of Alzheimer’s disease

Third-generation sequencing enables direct detection of genome-wide 5-methylcytosine without bisulfite conversion. Here, we present a protocol for generating long-read DNA sequencing from mouse cortex and performing genome-wide differential methylation analysis. We describe steps...

Accumulating safety data for remote ischemic conditioning in acute pediatric neurological conditions

Acute encephalopathy with biphasic seizures and late reduced diffusion (AESD) is the most common infection-triggered encephalopathy in East Asian children and one for which existing neuroprotective strategies are limited. Despite the use of therapeutic hypothermia (TH) or targete...

‘Africans Against Malaria’—introducing a new diaspora-led initiative to strengthen malaria prevention amongst UK-based travellers visiting friends and relatives in malaria-endemic African countries

Use of malaria prevention methods, particularly chemoprophylaxis, amongst the UK-based African diaspora is low; a long-standing issue that remains inadequately addressed. Here, we present ‘Africans Against Malaria’, a unique initiative ‘led by Africans, for Africans’ to improve m...

Validating the biological origin of in vivo fractional intracellular volume from VERDICT-MRI in the prostate

Abstract Objective Noninvasive microstructure estimates using magnetic resonance imaging (MRI) show potential in improving prostate cancer (PCa) diagnosis; however, histological validation is necessary. Materials and methods We used data from the Histo-MRI trial (NCT04792138). Pa...

Towards a deep-learning genomic tool for risk stratification and diagnostic support in sporadic ALS

Abstract Background A variety of common and rare genetic factors have been implicated in the development of amyotrophic lateral sclerosis (ALS), and the evidence is that a genetic component is present in most affected individuals. However, our current understanding of ALS genetic...

Pharmacological Inhibition of Small Extracellular Vesicle Secretion by ALK5i SD‐208 via Lysosomal Rerouting of CD63+ Compartments

ABSTRACT Pharmacological tools to selectively modulate extracellular vesicle (EV) secretion are scarce. Here, we identify the ALK5 (TGF‐β receptor I) inhibitor SD‐208 as a potent suppressor of small EV (sEV) secretion that acts independently of its canonical anti‐fibrotic activit...

Activity and participation after transient ischemic attack: a scoping review

Transient ischemic attack (TIA) is traditionally regarded as a condition with full recovery. However, emerging evidence suggests that some individuals experience residual symptoms, limiting their activity and participation in everyday life. The objective was to systematically map...

Data-Driven Fatigue Trajectories in Multiple Sclerosis and Their Predictors

Fatigue is one of the most disabling symptoms of multiple sclerosis (MS), yet its longitudinal trajectories and determinants remain poorly characterized. We aimed to identify trajectories of cognitive and physical fatigue in relapsing-remitting MS (RRMS) and to determine baseline...

Beta-lactam antibiotics have immunomodulatory effects in vitro, including changes consistent with sepsis-induced immunosuppression

Abstract Introduction Sepsis is associated with immunosuppression, predisposing patients to secondary infections. Many treatments routinely used for infections have immunomodulatory effects, including antibiotics. We therefore assessed the immunomodulatory effects of beta-lactam...