Thermo Fisher Scientific (Germany)

Extending single-cell proteomics from HeLa to small primary human immune cells

Abstract Single-cell proteomics (SCP) holds the promise of decoding cellular heterogeneity at the functional level, yet achieving deep and reproducible proteome coverage from individual cells has remained a formidable challenge. Here, we establish a high-sensitivity, label-free S...

Modulating pressure in the Orbitrap improves sensitivity and mass resolution in charge detection mass spectrometry

Abstract Native mass spectrometry has become a key method for studying macromolecular assemblies, providing insights into structures, stoichiometries, and binding interactions. A key aspect for the transmission of electrospray-generated bioparticles into the mass analyzer is the...