St Vincent's Clinic

Multiscale mechanical analysis of live breast cancer spheroids by micro‐compression and Brillouin imaging

Abstract Recent developments in cancer mechanobiology have highlighted the importance of tumour mechanics as a potential marker of disease progression. Three‐dimensional tumour models provide simplified yet physiological relevance exhibiting mechanical heterogeneity. However, com...

hpci improves reproducibility of computational biology research by integrating Continuous Integration with High Performance Computing clusters

Abstract Motivation The reproducibility crisis in bioinformatics and computational biology can be mitigated by using Continuous Integration (CI), a proven software engineering practice, to automate the testing of analysis pipelines and models. However, the adoption of CI in bioin...

Piezo1 footprint nanoarchitecture: from individual channels to collective behaviour and from outward to inward curvature

Piezo1 acts as a key architect of the cell membrane, generating a curved nanodome that extends beyond the protein trimer itself. Although Piezo1 intrinsically favours an outward (convex) membrane footprint, recent high-resolution studies have revealed that clustered channels can...

Beyond Publications: Why Dermatology Needs Research-on-Research (Meta-research)

The rise of articles published in biomedical and life sciences is staggering. The sheer numbers of biomedical publication make it impossible for a practising dermatologist to keep up with the literature and has ramifications for the entire medical publishing industry. Quantity do...

Opening threshold and kinetics of the MscL mechanosensitive channel are regulated by its periplasmic loop

Abstract Mechanosensitive (MS) channels are membrane proteins that respond to mechanical stimuli and are essential across prokaryotic and eukaryotic organisms. The E. coli mechanosensitive channel of large conductance (MscL) provides a powerful model for dissecting protein–lipid...

Oxygen-dependent subcellular redistribution of PHD3 links the hypoxic microenvironment to mitochondrial metabolic reprogramming in ccRCC

Abstract Clear cell renal cell carcinoma (ccRCC) is characterized by profound metabolic dysregulation, with both prolyl hydroxylase domain protein 3 (PHD3) and pyruvate carboxylase (PC) independently implicated in disease progression. Although each influences patient outcomes, a...

Vancomycin optimised dosing regimens for critically ill patients receiving renal replacement therapy

Optimal dosing of vancomycin in critically ill patients receiving renal replacement therapy (RRT) is uncertain due to high pharmacokinetic variability. We aimed to develop individualised vancomycin dosing recommendations that optimise efficacy while minimising toxicity. Prospecti...

Role of consolidative surgery after chemotherapy in recurrent/metastatic adrenal cortical cancer (ACC): Princess Margaret Cancer Centre experience

Recurrent/Metastatic (R/M) ACC is aggressive with few effective treatment options. The role of surgery after chemotherapy remains unclear. Moreover, prognostic factors in R/M ACC are not well defined. R/M ACC patients treated at Princess Margaret Cancer Centre (2002–2019) were re...