Second Hospital of Shandong University

Biomechanical comparison of two-, three-, and four-screw zero-profile ACDF constructs: effects of screw number and orientation

Zero-profile anterior cervical discectomy and fusion (ACDF) constructs vary in screw number and trajectory, but their effects on immediate stability and load transfer remain unclear. This study compared two-, three-, and four-screw constructs and examined middle-screw orientation...

Asymmetric Heterostructured Breathable Janus Nanofibrous Membranes for Deep Learning‐Assisted Wearable Sensing

ABSTRACT Long‐term skin‐interfaced wearable sensing requires integrated sweat management, breathability, thermal comfort, mechanical compliance, and stable signal acquisition, yet reconciling these requirements remains challenging in conventional homogeneous substrates or mechani...

Phloroglucinol inhibits human ureteral contractions via activation of cAMP‐PKA pathway

Abstract Background and Purpose Phloroglucinol is used to treat renal colic, especially when non‐steroidal anti‐inflammatory drugs are contraindicated. This study aimed to clarify its mechanism by examining effects on human ureteral contractility and underlying signalling pathway...

Design of Dual-Path Antibacterial Aluminum Alloy Based on ZnO Sputtering and Surface Roughness Control

Abstract Antibacterial alloys based on aluminum alloys have become a hot topic. However, most existing research is limited to a single antibacterial mechanism and may suffer from insufficient long-term efficacy, making it difficult to meet the requirements of large-scale producti...

Ammonia Accumulation Drives Intervertebral Disc Degeneration by Triggering Ammonia‐Induced Cell Death Through Lysosome–Mitochondria Crosstalk

ABSTRACT Intervertebral disc degeneration (IVDD) is a major cause of low back pain and is closely associated with metabolic imbalance within the disc microenvironment, yet the underlying mechanisms remain poorly understood. Here, we identify ammonia accumulation as a metabolicall...

IL-2-mediated Treg cells recruitment regulates neuroimmune crosstalk to promote hippocampal neurogenesis in depressive mice

Major depressive disorder (MDD) is a prevalent and debilitating neuropsychiatric disorder characterized by severe neuroinflammation and impaired hippocampal neurogenesis, yet effective clinical treatments remain limited. Accumulating evidence indicates that adaptive immune dysfun...

Discovery of Arginine-Reactive Covalent Kinase Inhibitors Overcoming Clinical Drug Resistance

Abstract Covalent kinase inhibitors (CKIs) serve as potent therapeutics, yet their development largely depends on conserved cysteine residues. Many kinases lack accessible cysteines or develop resistance mutations at these sites, limiting clinical utility. To diversify targetable...

Site-Specific Engineered Cationic Ferrocene COF with pH-Switchable Dual Enzyme Activities for Infected Wound Care

Abstract Infected wounds are trapped in self-perpetuating acidic and hypoxic microenvironments, yet designing intelligent systems that can sense and remodel such niches remains challenging. Here, we report EBP-Fc-COF, a cationically engineered ferrocene covalent organic framework...

Triglycerides and gestational diabetes mellitus: integrated clinical and genetic evidence linking LPL-related lipid metabolism

Abstract Background Gestational diabetes mellitus (GDM) is associated with adverse maternal and offspring outcomes, and disturbances in lipid metabolism may contribute to its metabolic pathophysiology. Elevated triglyceride (TG) levels have been consistently associated with GDM;...

Discovery and Synthesis of Selective Casein Kinase 1δ Degraders for Suppressing Oncogenic NRAS-Mutant Melanoma

Abstract Activating NRAS mutations occur in approximately 20% of melanomas but remain largely undruggable due to the lack of effective targeted therapies. We previously identified casein kinase 1δ (CK1δ) as a critical upstream regulator that stabilizes oncogenic NRAS mutants. How...

Therapeutic Cancer Vaccines: Classification, Mechanisms, Challenges and Future Perspectives

BACKGROUND: With the rapid advancements in oncology, immunology, and molecular biology, immunotherapy has emerged as a cornerstone of anti-tumor treatment, complementing traditional modalities such as surgery, radiotherapy, and chemotherapy. Therapeutic cancer vaccines are a prom...

Prognostic value of circ_0049271 in NSCLC and its regulatory effect on tumor

To explore the prognostic significance and functional role of circ_0049271 in non-small cell lung cancer (NSCLC). Clinical data were meticulously collected from 140 patients diagnosed with NSCLC. Lung cancer tissues, along with adjacent normal tissues, were preserved at -80 °C. A...

Bag-1 modulates lung ischemia-reperfusion injury by regulating BCL-2 family proteins and IP3R-mediated calcium release

Lung ischemia-reperfusion injury (LIRI) involves dysregulated apoptosis and calcium overload. This study explored the role of molecular chaperone BCL-2-associated athanogene 1 (Bag-1) -1 in LIRI pathogenesis. Using an A549 cell model of glucose deprivation/hypoxia-reoxygenation,...

Changes in frailty and incident circadian syndrome in middle-aged and older adults: a nationwide prospective cohort study

Circadian syndrome (CircS) is more predictive than metabolic syndrome (MetS). However, few studies have examined its risk factor. This study aims to investigate whether the static state or dynamic change of frailty is correlated with incident CircS in Chinese adults. Data were ob...

CD80-targeted songorine nanotherapy restores PINK1-dependent mitophagy in proinflammatory macrophages for osteoarthritis treatment

Synovial macrophage-mediated inflammation and mitochondrial dysfunction are increasingly implicated in osteoarthritis (OA) progression, yet targeted restoration of mitochondrial quality control remains challenging. Here, we developed a macrophage-targeted, targeted delivery nanop...

Potential roles of short-chain fatty acids in chronic obstructive pulmonary disease from the gut-lung axis perspective: an exploratory trend summary and discussion on biological mechanisms based on current evidence

Short-chain fatty acids (SCFAs) have been proposed as mediators of the gut-lung axis in chronic obstructive pulmonary disease (COPD). However, the human evidence is limited and heterogeneous across disease states and specimen types. We therefore synthesized the available evidence...

Neoadjuvant Anbenitamab and HB1801 in ERBB2 -Positive Breast Cancer

IMPORTANCE The combination of neoadjuvant taxanes with trastuzumab and pertuzumab remains the cornerstone treatment strategy in ERBB2 -positive breast cancer. Anbenitamab (a ERBB2 -biparatopic antibody that induces profound receptor clustering) and HB1801 (a solvent-free albumin-...

Hepatocyte Hedgehog activation attenuates acetaminophen-induced liver injury through a Vegfa-associated vascular response

Abstract Acetaminophen (APAP) overdose is the leading cause of drug-induced acute liver failure, yet early pathways linking hepatocellular stress to tissue integrity remain incompletely defined. Here, we demonstrate that canonical Hh signaling is rapidly activated in centrilobula...

Perception, coping, and needs of frailty in older patients with COPD: a qualitative study from patient, caregiver, and healthcare provider perspectives

Multidimensional frailty substantially affects health outcomes in older adults with chronic obstructive pulmonary disease (COPD); however, its comprehensive management remains insufficiently investigated. Existing research has largely focused on physical frailty from the patient...

The role of SAM-dependent internal m7G modification of LINC01252 in the progression of hepatocellular carcinoma

Abstract Dysregulation of long non-coding RNAs (lncRNAs) plays a significant role in the progression of hepatocellular carcinoma (HCC), yet many underlying mechanisms remain elusive. Recent studies have highlighted the critical importance of internal N 7 -methylguanosine (m 7 G)...