Reial Acadèmia de Ciències i Arts de Barcelona

Donor‐induced conformational gating and substrate‐assisted catalysis in α ‐1,3‐galactosyltransferase

Retaining glycosyltransferases catalyze the formation of stereochemically conserved glycosidic bonds through mechanisms that remain debated. Using bovine α1,3-galactosyltransferase (α3GalT) as a model, we combine mutagenesis, equilibrium unfolding, kinetics, and molecular dynamic...