Neuroscience Institute Cavalieri Ottolenghi

Computational identification and experimental validation of early glycinergic impairments in spinal muscular atrophy

Spinal Muscular Atrophy (SMA) is a progressive neurodegenerative disorder characterized by lower motor neuron (MN) loss, due to mutations or deletions in the Survival Motor Neuron 1 ( SMN1 ) gene. Increasing evidence implies mitochondrial dysfunctions in SMA, suggesting these org...

Modeling Diabetic Neuropathy: Injury Biomarkers for Early Detection Through Neuronal and Glial In Vitro Models

Diabetes mellitus (DM) is a rapidly expanding global health burden and a leading cause of chronic complications. Among these, diabetic peripheral neuropathy (DN) is one of the most prevalent and disabling, affecting up to half of individuals with long-standing diabetes and involv...

Volume Electron Microscopy of Cortical Organoids: Methods for Region Identification, Connectome Reconstruction, and Organelle Segmentation

Abstract Volume electron microscopy (vEM) has become a powerful tool for 3D ultrastructural analysis of neural circuits, yet its application to human brain organoids remains limited, particularly for connectomic studies. Here, we established a comprehensive and scalable workflow...