National Horticultural Research Institute

Disruption of Male Spodoptera litura Pheromone Attraction by Structurally Related Compounds: Electrophysiological and Field Evidence

The tobacco cutworm, Spodoptera litura, is a globally important agricultural pest that relies on a female-produced sex pheromone blend for mate location. Here, we investigated the electrophysiological and behavioral responses of S. litura to its sex pheromone components and struc...

Molecular identification of microorganisms: identification of microorganisms cultivated on microbiological media based on the analysis of gene sequences

This technique allows identification of genus/species of microorganisms based on the sequence of several, usually seven, selected loci, with the analysis involving the sequences of those genes that determine the basic functions of the cell. The data collected on the basis of sequ...

Molecular identification of microorganisms: identification of microorganisms cultivated on microbiological media based on the analysis of gene sequences

This technique allows identification of genus/species of microorganisms based on the sequence of several, usually seven, selected loci, with the analysis involving the sequences of those genes that determine the basic functions of the cell. The data collected on the basis of sequ...

Assessment of the survival rate of microorganisms in the soil and assessment of the soil microbial biodiversity with the use of the DGGE technique

Only a small proportion of microorganisms living in the soil environment start growing on nutrient media in the laboratory. For this reason, molecular techniques based on the analysis of DNA extracted directly from the soil are used to study the microbial diversity of soils. Thes...

Assessment of the survival rate of microorganisms in the soil and assessment of the soil microbial biodiversity -soil sampling

The application of beneficial microorganisms to the soil requires the control of their survival. The DGGE technique (Denaturating Gradient Gel Electrophoresis) is used for this purpose. The first step in the DGGE analysis is to collect soil samples.

The CmMYB6–CmWRKY13–CmBr module mediates cucurbitacin B accumulation in melon fruit under CPPU treatment

ABSTRACT Cucurbitacin B (CuB), a key compound responsible for the bitterness of melon fruit, significantly reduces fruit quality and consumer acceptance. Therefore, understanding the molecular mechanism underlying CuB biosynthesis is essential for improving melon fruit quality. I...

UAV-based YOLOv11 system for automated detection and counting of male hemp plants bearing staminate flowers

Abstract The flowering stage is a crucial phenological phase in hemp production, determining the maturity group of grain and fiber varieties, yield potential, and optimal harvest timing. Enabling early-stage detection of flowering male plants and distinguishing them from non-flow...