National Center for Biotechnology

Synthetic horizontal gene transfer for ecosystem restoration

Abstract Restoring endangered ecosystems has become a pressing issue as the effects of global warming continue to harm existing communities. During the last decade, several studies have suggested the use of synthetic biology as a tool to protect these biodiverse communities by in...

Recombinant Thermostable DNA Polymerases: Current Approaches to Production, Molecular Engineering, and Applications in Biotechnology and Diagnostics

DNA polymerases are crucial for the replication and repair of genetic material. Advances in recombinant DNA technology and protein engineering have enabled the production of enzymes with specific catalytic properties tailored to the requirements of molecular diagnostics, next-gen...

Multiplex RPA-CRISPR/Cas12a Assay for Rapid Detection of Class D OXA-Type Carbapenem-Resistant Acinetobacter baumannii

Acinetobacter baumannii is a critical WHO priority pathogen due to its multidrug resistance and high mortality in carbapenem-resistant infections. Resistance is predominantly mediated by class D carbapenemase genes blaOXA-23 and blaOXA-40, which spread rapidly via horizontal gene...