National Center for Biotechnology

Steppe-Derived Glyphosate-Tolerant Pseudomonas sp. ADL-13 and Paenibacillus sp. ZHS-9 Strains with Plant Growth-Promoting Traits and Broad Abiotic Stress Tolerance

The integration of plant growth-promoting bacteria (PGPB) into sustainable agricultural practices represents a promising strategy for improving crop productivity and stress resilience. We characterized two newly isolated steppe-derived, glyphosate-tolerant bacteria, Pseudomonas s...

Recombinant Expression and Functional Analysis of GH2 β-Galactosidases Derived from Lactobacillus delbrueckii subsp. bulgaricus NSP1 and Streptococcus thermophilus RB16

GH2 β-galactosidases are important industrial biocatalysts for lactose hydrolysis and prebiotic galacto-oligosaccharide (GOS) synthesis. Here, lacZ genes from Streptococcus thermophilus RB16 (StLacZ) and Lactobacillus delbrueckii subsp. bulgaricus NSP1 (LbLacZ) were cloned, seque...

Genetic Basis of Social Structure in the Pastoral Nomads of Central Eurasia

Abstract Although the social structure of Central Eurasian pastoral nomads has been described as fluid and ad hoc by historians and anthropologists, its potential genetic basis remains poorly understood. To evaluate whether kinship based social organization has biological foundat...

Eleven-Year Trends in Stillbirth, Neonatal and Infant Mortality and Birthweight-Specific Risk: A BABIES Matrix Analysis from a Tertiary Maternity Center in Kazakhstan, 2015–2025

Background/Objectives: Long-run facility-level perinatal data from Central Asia are scarce. We described eleven years of stillbirth, neonatal, infant and perinatal mortality at one tertiary maternity center in Kazakhstan, and used the Birthweight and Age-at-death Boxes for Interv...

Physiological, Biochemical, and Molecular Responses of Potato Cultivars to Osmotic Stress Under In Vitro Shoot Culture

Drought is a major limiting factor for potato productivity. This study examined two Kazakh potato cultivars, Tokhtar and Aksor, under osmotic stress induced by polyethylene glycol (PEG)-6000 using an integrated physiological, biochemical, and molecular approach. We evaluated plan...

Defect engineering via post-annealing in Cu-doped ZnO thin films: Impact on optical and antibacterial performance

Copper-doped zinc oxide (ZnO:Cu) thin films were deposited by vacuum thermal evaporation then annealed at 300°C, 400°C, and 500°C in order to tailor defect states and surface-related functional properties. X-ray diffraction analysis revealed a transition from an amorphous structu...

Synthetic horizontal gene transfer for ecosystem restoration

Abstract Restoring endangered ecosystems has become a pressing issue as the effects of global warming continue to harm existing communities. During the last decade, several studies have suggested the use of synthetic biology as a tool to protect these biodiverse communities by in...

Recombinant Thermostable DNA Polymerases: Current Approaches to Production, Molecular Engineering, and Applications in Biotechnology and Diagnostics

DNA polymerases are crucial for the replication and repair of genetic material. Advances in recombinant DNA technology and protein engineering have enabled the production of enzymes with specific catalytic properties tailored to the requirements of molecular diagnostics, next-gen...

Multiplex RPA-CRISPR/Cas12a Assay for Rapid Detection of Class D OXA-Type Carbapenem-Resistant Acinetobacter baumannii

Acinetobacter baumannii is a critical WHO priority pathogen due to its multidrug resistance and high mortality in carbapenem-resistant infections. Resistance is predominantly mediated by class D carbapenemase genes blaOXA-23 and blaOXA-40, which spread rapidly via horizontal gene...