Max Planck Institute for Developmental Biology

An evolutionarily conserved N-terminal domain of RRF-3 governs GTSF-1 binding in nematodes

Abstract GTSF1 is an essential activating cofactor for PIWI proteins in many metazoans. In the nematode Caenorhabditis elegans , however, GTSF-1 does not bind PIWI, but is associated with the RNA-dependent RNA polymerase RRF-3, supporting endo-siRNA (26G-RNA) biogenesis. Here, we...