Joint BioEnergy Institute

An automated operon refactoring workflow for heterologous expression of non-ribosomal peptide biosynthetic gene clusters

Microbial genomes encode numerous biosynthetic gene clusters, yet most remain experimentally uncharacterized, particularly large non-ribosomal peptide synthetase (NRPS) clusters that resist conventional cloning. Existing refactoring approaches are labor-intensive, depend on de no...

The rice cellulose synthase-like D4 gene ( OsCSLD4 ) is required for resistance to Xanthomonas pv. oryzae

Plant cell walls serve as a physical support and a barrier to pathogen invasion. Cellulose is the main component of cell walls. The cellulose synthase-like D ( CSLD ) subfamily genes are required for plant normal development. In rice, Oscsld4 mutant plants are dwarfed and have na...

Ambient Alkaline Pretreatment Enables Isoprenol Fermentation from Lignocellulosic Biomass for Sustainable Aviation Fuel Production

Abstract Mitigating aviation’s carbon emissions remains challenging because air travel relies on energy-dense liquid fuels with limited substitutes. Here, we report a fermentation-based bioconversion process for producing isoprenol, a precursor to 1,4-dimethylcyclooctane (DMCO),...

Ensemble agroecosystem modeling enhances predictions of crop yields and soil carbon across the United States

Abstract. Accurately estimating crop yields and soil organic carbon (SOC) dynamics is essential for agricultural planning, carbon accounting, and sustainable land management. However, process-based agroecosystem models often produce divergent estimates due to variations in model...