Intelligent Synthetic Biology Center

Biocatalytic Synthesis of dNTPs from Bacterial Biomass

Abstract Deoxyribonucleoside triphosphates (dNTPs) are crucial reagents widely utilized in nucleic acid amplification. However, current synthesis methods are often plagued by environmental pollution for chemical synthesis and the high costs of phosphate donors for enzymatic synth...

Semi-automated strain engineering and screening in the thermophilic fungus Myceliophthora thermophila for recombinant protein production

ABSTRACT Thermophilic filamentous fungi are widely used for cellulase production at elevated temperatures; however, efficient recombinant protein production in these organisms remains limited because construction and screening of large transformant populations remain difficult. H...

Chemo-Enzymatic Synthesisof Purine 3′-O-Amino-DeoxyribonucleosideTriphosphates

Abstract 3′-O-amino-deoxyribonucleoside triphosphates (3′-ONH2-dNTPs) are crucial reversible terminators for template-independent DNA synthesis. Owing to the structural complexity of purine bases, here we propose a chemo-enzymatic process for the synthesis of purine-type 3′-ONH2-...