Instituto Butantan

Two new species of Callidosomatinae Southcott, 1961 (Trombidiformes: Erythraeidae) from the Carajás region, eastern Brazilian Amazon

The diversity of Callidosomatinae in the Neotropical region remains poorly known, particularly in megadiverse ecosystems such as the Amazon. In this study, two new species of Callidosomatinae, belonging to the genera Callidosoma and Iguatonia, are described based on specimens col...

Mucin- and mucus-related phenotypes and in vitro interaction of extraintestinal pathogenic Escherichia coli with human pulmonary cells

Abstract Background Extraintestinal pathogenic Escherichia coli (ExPEC) is a major cause of urinary tract infections (UTI), bacteremia, and meningitis. Although ExPEC have also been associated with pneumonia, the virulence mechanisms that enable colonization and infection remain...

Nuclear proteome analysis suggests spatial and temporal compartmentalization of metabolic enzymes during Trypanosoma cruzi cell cycle

Trypanosoma cruzi , the etiological agent of Chagas Disease (CD), represents a significant public health concern and serves as a valuable model for investigating the cell cycle in early-diverging eukaryotes. Its unique cellular features,including the absence of chromosome condens...

Unraveling the Glycosylation Machinery of Bothrops jararaca Snake Through Multiomics and Structural In Silico Analyses

Glycosylation is a ubiquitous post-translational modification (PTM) that influences folding, stability, and function of proteins. In eukaryotes, N-glycosylation corresponds to the linkage to asparagine (Asn), while O-glycosylation involves the linkage to serine (Ser) or threonine...

Development of an Electrochemical Reactor Prototype for Biodiesel Production Powered by Photovoltaic Energy

Abstract The continuous increase in global energy demand has intensified the search for renewable alternatives capable of replacing fossil fuels. In this study, an electrochemical reactor prototype powered by photovoltaic energy was developed for biodiesel production from sunflow...

Integrated Genome Mining and Bioactivity-Guided Isolation of Antimicrobial Peptides from Bacillus amyloliquefaciens BS4

Bacterial resistance remains a critical global health challenge, driving the continuous search for novel antimicrobial agents. Bacillus amyloliquefaciens is a recognized repository of bioactive metabolites; however, its full biosynthetic potential requires integrated genomic and...

Sexual Dimorphism and Intraspecific Toxin Variation in Crotalus durissus terrificus Venom: Insights from Proteomics, Peptidomics, and Structural Modeling

Abstract The venom of the South American rattlesnake Crotalus durissus terrificus (Cdt) is renowned for its minimalist yet highly toxic arsenal. Intraspecific variability in expressed proteoforms and toxin content has been previously observed in Cdt venom, resulting in heterogene...

Sialotranscriptome of Simulium pertinax and functional characterization of SpVaP: a novel recombinant simuliid-specific vasodilator

Abstract The salivary glands of hematophagous arthropods possess a remarkable molecular diversity, reflecting evolutionary adaptations to blood feeding lifestyle and vector-host interactions. In this study, we characterized the salivary-gland transcriptome of adult female Simuliu...

Delivery of multiple PspA fragments by CyaA toxoid induces broad protection against Streptococcus pneumoniae

Abstract Streptococcus pneumoniae is a leading cause of lower respiratory tract (LRT) infections and invasive pneumococcal diseases (IPD). Conjugate vaccines are effective against IPD and nasal colonization but offer coverage limited to serotypes included in the formulations. Pne...

Albumin Precoating of Nanoparticles: A Reliable StealthStrategy?

Abstract Albumin precoating has emerged as a strategy to impart nanoparticles (NPs) with stealth-like properties and as an alternative to NP surface PEGylation. Yet how such coatings influence protein corona formation and hemocompatibility in human blood remains poorly understood...

cAmbly, a peptide derived from the star tick Amblyomma sculptum salivary gland transcriptome, attenuates inflammatory pain by activating endogenous opioid pathways

Pain affects more than 1.5 billion people worldwide and remains a leading cause of disability, but currently available analgesics often have limited efficacy and significant adverse effects. In this context, bioactive molecules derived from animal venoms and secretions have emerg...

Reduced IP6K levels impair life-cycle transitions and intracellular development of Trypanosoma cruzi within human cardiomyocytes

Trypanosoma cruzi is the etiological agent of Chagas disease (CD). During its life cycle, T. cruzi undergoes several key differentiation processes that are essential for its survival. The precise mechanisms that regulate these processes remain elusive, and any interference in thi...

Acidic StressInduces Proteomic Reprogramming andVirulence-Associated Adaptation in Paracoccidioides brasiliensis

Abstract Paracoccidioidomycosis (PCM) is a neglected systemic mycosis whose etiologic agents must adapt to acidic host niches such as phagolysosomes. Here, we used quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics to define the response of Pa...