Institute of Plant Genetics, Polish Academy of Sciences

Efficient in-vitro regeneration and transformation for CRISPR/Cas9-mediated genome editing of phytoene desaturase (PDS) gene in pea (Pisum sativum L.)

Key message The present study addresses optimization of in-vitro regeneration via direct organogenesis and Agrobacterium-mediated genetic transformation, enabling efficient multiplex CRISPR/Cas9-based genome editing of the phytoene desaturase (PsPDS) gene in pea. Abstract Pea (Pi...

Co-option of ancestral stem regulators drove recurrent evolution of underground storage organs

Geophytes are plants that produce underground storage organs such as tubers, rhizomes, and bulbs, to facilitate asexual reproduction and withstand a myriad of environmental challenges. While the potato ( Solanum tuberosum L.) serves as the primary model for studying tuberization,...

Nitrate bioaccumulation, growth performance and survival of rainbow trout ( Oncorhynchus mykiss ) in aquaponics with elevated water NO 3 -N levels

Aquaponics systems offer a sustainable method for the combined production of fish and plants, but they may lead to elevated nitrogenous compounds in water to satisfy plant nitrogen requirements. However, limited information is available on nitrate accumulation in fish flesh under...