Institute of Genomics and Integrative Biology

Cyclodextrin-enabled δ-Tocotrienol for Treating Stress and Memory Dysfunction: Molecular Insights, Biotechnological Applications and Biomanufacturing Implications

Background Antioxidants possess substantial health benefits owing to their ability to alleviate cellular oxidative stress, reactive oxygen species generation and inflammation. Vitamins, dietary fatty acids, minerals, micronutrients and plant-derived flavonoids have attracted sign...

Small Extracellular Vesicle-Associated Chromosome 14 microRNA Cluster Members Reveal Distinct Molecular Responses to E-Liquid Exposure in Human Lung Cells

Background/Objectives: The adoption of electronic cigarettes and vaping devices worldwide as healthier alternatives to smoking tobacco has led to a new clinical condition termed EVALI—E-cigarette or vaping-associated lung injury. The symptoms of EVALI present large overlaps with...

LC3 forms functional nanoclusters on autophagosomes

Abstract Autophagosome biogenesis depends on the coordinated action of proteins and lipids. However, how LC3B organizes at high density and contributes to autophagy-associated functions remains unclear. Using molecular dynamics simulations and super-resolution microscopy, we show...

Minimal organism JCVI-syn3.0, an engineered derivative of Mycoplasma mycoides , encodes an assortment of unrecognized membrane transporters and immunity factors

ABSTRACT In 2016, researchers at the J. Craig Venter Institute produced JCVI-Syn3.0, a derivative of Mycoplasma mycoides subsp. capri Large Colony strain GM12 with a re-engineered, minimized genome. The minimal synthetic genome of 532 kbp encodes 473 genes, selected from ∼910 par...

Crocetin HaltsTumorigenesis in Metastatic BreastCancer via Disrupting CYP2J2/EET Axis

Abstract CYP2J2 catalyzes the conversion of arachidonic acid into epoxyeicosatrienoic acids (EET), which have gained significant attention due to their oncogenic properties. Differential overexpression of CYP2J2 in tumors results in increased EET production. The role of EET in tu...

Identification and characterization of PldB domain-containing esterase from a freshwater pond metagenome

Esterases, a class of α/β-hydrolases, are crucial enzymes used for the biotransformation of industrially important biomolecules. Exploring the pond metagenome, a lipolytic clone. (PLR5) was identified that exhibited activity against tributyrin. This clone encodes a unique putativ...

Energetic Determinantsof Expanded PAM Recognitionin Engineered FnCas9

Abstract Expanding protospacer-adjacent motif (PAM) compatibility while preserving specificity remains a central challenge in CRISPR–Cas9 engineering. Francisella novicida Cas9 (FnCas9) exhibits high intrinsic specificity but is constrained by stringent PAM requirements. Here, we...