Institute of Genomics and Integrative Biology

Crocetin HaltsTumorigenesis in Metastatic BreastCancer via Disrupting CYP2J2/EET Axis

Abstract CYP2J2 catalyzes the conversion of arachidonic acid into epoxyeicosatrienoic acids (EET), which have gained significant attention due to their oncogenic properties. Differential overexpression of CYP2J2 in tumors results in increased EET production. The role of EET in tu...

Energetic Determinantsof Expanded PAM Recognitionin Engineered FnCas9

Abstract Expanding protospacer-adjacent motif (PAM) compatibility while preserving specificity remains a central challenge in CRISPR–Cas9 engineering. Francisella novicida Cas9 (FnCas9) exhibits high intrinsic specificity but is constrained by stringent PAM requirements. Here, we...

Identification and characterization of PldB domain-containing esterase from a freshwater pond metagenome

Esterases, a class of α/β-hydrolases, are crucial enzymes used for the biotransformation of industrially important biomolecules. Exploring the pond metagenome, a lipolytic clone. (PLR5) was identified that exhibited activity against tributyrin. This clone encodes a unique putativ...