Institute of Gene Biology

Delivery of transgenes into Gαo-rich subcortical structures of the mouse brain with potential applications for GNAO1 encephalopathy

Adeno-associated virus (AAV) vectors are widely used for transgene delivery into the mouse brain in preclinical studies, yet AAV methodology requires customization for each neurological disease. In this study, we aimed to identify the optimal approach for AAV-mediated transductio...

Identification of Highly Cytotoxic Antisense Oligonucleotides Targeting Essential Genes in Human Ovarian and Lung Cancer Cells

Therapeutic nucleic acid (TNA)‐based gene silencing strategies, such as antisense oligonucleotides (ASOs), have been explored for cancer treatment. However, no ASO‐based anticancer drugs are currently available, largely because of limited therapeutic efficacy. Conventional ASO‐ba...

The sensitivity of acute myeloid leukemia to CDK8/19 inhibitors is determined by their metabolic profile

Cyclin-dependent kinases CDK8/19 are serine/threonine kinases that regulate transcription as part of the Mediator complex and phosphorylate several non-transcriptional substrates in both normal and tumor cells. Several studies have demonstrated that inhibition of CDK8/19 leads to...

Multi-level aggregation analysis of microbiome composition and host gene expression reveals associations with systemic and local immunity

ABSTRACT The human gut microbiome plays a critical role in immune regulation, yet the molecular links between microbiome composition and host gene expression remain incompletely understood. We analyzed associations between host gene expression and microbiome composition in a coho...