Institute of Bioorganic Chemistry

Application of Machine Learning Methods for Processing and Analyzing Data from Probe and Optical Microscopy of Biological Objects

Abstract This review systematizes modern machine learning (ML) methods for solving key image processing tasks in optical microscopy (OM) and atomic force microscopy (AFM). Traditional microscopy data analysis methods face problems of subjectivity, low throughput, and difficulty i...

NMR Structure and Dynamics of the Starfish Three-Finger Protein Lystar5

Abstract Objective: The primary goal of this study was to determine the spatial structure and investigate the intramolecular dynamics of Lystar5, the first functionally characterized three-finger protein (TFP) from the starfish Asterias rubens. Methods: Spatial structure determin...

Mycobiota of the Rhizosphere of Vaccinium Shrubs in a Pine Forest in the Early Months after a Ground Fire

Abstract Ground fires are a frequent phenomenon in boreal forests. Scotch pine (Pinus sylvestris), as a major climax species, plays a key ecological role within these ecosystems. The soil and rhizosphere microbiome exerts a significant influence on the postfire recovery of pine f...

Development of a Highly Efficient System for the Production of the Holoform of Recombinant Cytoglobin

Abstract Objective: A highly efficient production system for recombinant human cytoglobin using minimal medium has been developed and optimized. This system enables the production of the water-soluble holoform of the protein—in both unlabeled and isotopically labeled forms—in qua...

The Effect of Mutations in Glycanase Genes on the Formation of Exo-oligosacharides in Nodule Bacteria Rhizobium leguminosarum bv. viciae VF39

Abstract In order to identify the mechanisms underlying the formation of low-molecular-weight forms of acidic exopolysaccharide in the symbiotic bacteria Rhizobium leguminosarum bv. viciae, a comparative analysis of exo-oligosaccharide production was conducted in the wild-type st...

pCORE, a set of open modular plasmids compatible with golden gate assembly

Abstract Modular DNA assembly based Golden Gate cloning (MoClo) is a widely used framework to assemble genetic constructs. A number of MoClo “destination vectors” have been developed, however, available options are still limited with regards to copy number and antibiotic resistan...

Ring Contractionof Bicyclic Lactams: A MolecularEditing Aza-Favorski-Type Approach to Tetrahydroquinoline-2-carboxylicAcids

Abstract Development of skeletal editing methods offers new horizons for the acceleration of drug discovery. An original aza-Favorski-type approach is described for the ring contraction of benzazepine-2-ones, providing versatile tetrahydroquinoline-2-carboxylic acids. The sequenc...

Linker Engineering of Hybrid Triazole-Thiazolidine Antifungals Identifies a Promising Lead Against Drug-Resistant Candida Species

Background: The emergence of antifungal resistance and the limited number of clinically available antifungal drug classes necessitate the development of new agents with improved efficacy and safety. We investigated how linker architecture influences the antifungal activity and le...