Institute of Bioinformatics

N-linked glycoproteomic and proteomic characterization of esophageal squamous cell carcinoma cell lines

Esophageal squamous cell carcinoma (ESCC) is an aggressive disease with a low survival rate. While considerable progress has been made in uncovering the biological mechanisms of the disease, diagnosing ESCC at an early stage remains challenging. Altered N-glycosylation patterns a...

DyME: An MD-based engine exploiting HTP mutagenesis for protein engineering and recognition mimicry

Protein recognition mimicry is of great interest in the field of molecular bioengineering and rational design, with mutagenesis frequently employed to analyze the effects of altering amino acids involved in molecular recognition. The conformational and energetic effects of such a...

Antimicrobial resistance in functional fruits-associated lactic acid bacteria within agri-food systems

Antimicrobial resistance (AMR) in fermented food-origin Lactic Acid Bacteria (LAB) has raised significant concern for food safety. However, AMR in the LAB of unfermented functional fruits consumed raw or minimally processed remains understudied. Here, two hundred sixty samples of...

Hex -MASP for mapping the whole-tissue spatial proteome and the intrabrain distribution of monoclonal antibodies

Abstract Whole-tissue spatial proteomics level provides critical insights into region-specific biological regulations but remains challenging. Previously, we introduced the Micro-scaffold Assisted Spatial Proteomics (MASP) concept for whole-tissue mapping. However, this prototype...

Synthesis, Anticancer Evaluation, mRNA Expression Analysis, and Molecular Docking of Fluorinated Picolinamide–Benzothiazole Derivatives Against Breast Cancer‐Related Genes CDK2, ERK2, and BCL2

ABSTRACT The present study focuses on the design, synthesis, characterization, and biological evaluation of two new series of derivatives 4‐(4‐amino‐3‐fluorophenoxy)‐N‐methylpicolinamide ( 3a–e ) and 2‐aminobenzothiazole ( 5a‐e ) as potential antioxidant and anticancer agents. Th...

Reduced IP6K levels impair life-cycle transitions and intracellular development of Trypanosoma cruzi within human cardiomyocytes

Trypanosoma cruzi is the etiological agent of Chagas disease (CD). During its life cycle, T. cruzi undergoes several key differentiation processes that are essential for its survival. The precise mechanisms that regulate these processes remain elusive, and any interference in thi...

Systems biology analysis reveals miRNA–mRNA networks associated with cardiomyocyte responses to SARS-CoV-2 infection

Cardiac complications are common and clinically significant in COVID-19, yet their underlying molecular drivers remain poorly understood. Here, we integrate post-mortem histopathology with transcriptomic and microRNA (miRNA) analyses to delineate the regulatory architecture of SA...