Institute of Biochemistry

Mechanistic insights into the inhibition of insulin amyloidogenesis and associated toxicity by the p. M14L variant of exenatide

Insulin fibrillation and the subsequent formation of toxic amyloid aggregates are significant challenges in therapeutic development and are associated with cellular proteotoxicity. In this research, we explored how the p.M14L exenatide variant affects the formation of insulin amy...

Protocol for cyclic DNA-damaging treatments to generate high mutational burden in the human A549 cell line

Here, we present a protocol that describes iterative exposure of the human lung cancer cell line A549 to DNA-damaging agents to progressively increase mutational burden. We describe steps for controlled cytotoxic dosing, metabolic activation, and repeated treatment cycles of up t...