Institut de génétique et de biologie moléculaire et cellulaire

Sensitive reverse transcription-digital droplet PCR-based discrimination of hepatitis delta virus RNAs reveals differential effects of interferon-α on viral RNA species

Hepatitis delta virus (HDV) replication relies on the production of distinct viral RNA species, including genomic (HDV-G), antigenomic (HDV-AG) and mRNAs (HDV mRNAs), which differ in abundance and function. However, the lack of sensitive and quantitative approaches allowing their...

Pro-apoptotic polymers for neutrophil-induced inflammation modulation: from screening to therapeutic potential

Human polymorphonuclear neutrophils (PMNs) are pivotal in the initiation and resolution of inflammation, playing an essential role in maintaining tissue homeostasis and facilitating repair. This study investigates how polymers influence PMNs apoptosis, a favourable step for the r...

KMT9 drives T cell exclusion and dysfunction by promoting PMN-MDSCs infiltration and ARG1 expression in prostate cancer

Immunotherapy has emerged as a revolutionary therapeutic approach to treat cancer showing remarkable clinical responses. However, its efficacy in solid tumours such as prostate cancer (PCa) remains very limited due to a highly immunosuppressive tumour immune microenvironment (TIM...

Androgen receptor imprints satellite cell stemness and preserves their reservoir for lifelong regeneration and optimal repair

Skeletal muscle stem cells (MuSC) are the guardians of muscle regeneration, sustaining tissue integrity through a delicate balance of quiescence, activation, and lineage commitment. While numerous molecular cues have been implicated in regulating these processes, the influence of...

Clinical Variability and Genotype‐Driven Outcomes in CHRND ‐Related Congenital Myasthenic Syndrome

BACKGROUND: Congenital myasthenic syndromes (CMS) caused by pathogenic variants in CHRND, encoding the δ-subunit of the nicotinic acetylcholine receptor (AChR), are rare, and data on genotype-phenotype correlations and long-term outcomes are limited. METHODS: We performed a retro...

Distinct Molten‐Globule Conformations in an Interacting Protein Domain Resolved by 19 F NMR of Fluoroproline Residues

ABSTRACT Recently, the application of deep learning to structural data deposited in the Protein Data Bank has enabled the reliable and accurate prediction of 3D folded structures of proteins from their sequences. However, this approach is not applicable to highly dynamic proteins...

Distinct Molten‐Globule Conformations in an Interacting Protein Domain Resolved by 19 F NMR of Fluoroproline Residues

ABSTRACT Recently, the application of deep learning to structural data deposited in the Protein Data Bank has enabled the reliable and accurate prediction of 3D folded structures of proteins from their sequences. However, this approach is not applicable to highly dynamic proteins...

Longitudinal progression, metrics, age-dependence, and modifiers of ataxia severity in SCA27B: a multicentre study of 219 patients

Background Spinocerebellar Ataxia 27B (SCA27B) is a novel, frequent and likely treatable late-onset autosomal-dominant ataxia caused by GAA repeat-expansions in FGF14 . For understanding disease evolution and imminent trial planning, metrics of the most widely used clinical outco...

FGFR3-driven gene regulatory network analysis reveals a protumoral role for p63 in luminal bladder tumors

Fibroblast growth factor receptor 3 ( FGFR3 ) is one of the most frequently altered genes in bladder cancer, primarily through activating mutations that drive oncogenesis and are enriched in luminal tumors. However, the underlying gene regulatory network (GRN) remains poorly char...