HUN-REN Szegedi Biológiai Kutatóközpont

Fungal frontiers for sustainability: unlocking the hidden kingdom

Fungi have repeatedly delivered transformative advances across industries, from medicine to food to materials, yet these breakthroughs have emerged from a narrow base of organisms and well-studied systems. Although mycology has taken a big leap forward in the past decades because...

Protocol for cyclic DNA-damaging treatments to generate high mutational burden in the human A549 cell line

Here, we present a protocol that describes iterative exposure of the human lung cancer cell line A549 to DNA-damaging agents to progressively increase mutational burden. We describe steps for controlled cytotoxic dosing, metabolic activation, and repeated treatment cycles of up t...

Transient Absorption Spectroscopy of the Stilbenes in Methanol─Adiabatic cis→ trans Photoisomerization by Sequential Two-Photon Excitation

Abstract Femtosecond pulse excitation gave the time evolution of the transient absorption spectra (TAS) of trans- and cis-stilbene (t- and c-St) in methanol. Global analysis of t-St TAS gives the spectrum of the singlet excited state of t-St, 1t*, with a lifetime, 46 ps, consiste...

Multiple overlapping SNARE complexes drive endosome maturation in Drosophila nephrocytes

ABSTRACT Endosomal maturation determines whether internalized cargo is recycled or degraded, yet the molecular logic governing early endosomal fusion remains poorly defined. This process is often depicted as a linear Rab5-to-Rab7 transition mediated by a single, ordered SNARE pat...

flexiAsCas12a and other Cas12a variants enhance the applicability of Cas12a nucleases in prime editing

Abstract Background Amongst the genome manipulation tools based on the versatile CRISPR-Cas system, prime editing is the most prominent one as a method that enables precise insertions, deletions, and substitutions without inducing double-strand breaks. Cas12a nucleases are widely...