Hubei University of Chinese Medicine

Follistatin-Like Protein 1 Contributes to Acute Traumatic Spinal Cord Injury by Enhancing Neuroinflammation Through the Modulation of Interferon Regulatory Factor 5-Mediated Macrophage Activation

Background and Purpose: Follistatin-like protein 1 (Fstl1) is a secreted glycoprotein with context-dependent inflammatory activity. We investigated whether Fstl1 is a reproducible feature of traumatic spinal cord injury (SCI) and whether it regulates macrophage activation through...

Effects of various exercise interventions in adults with metabolic syndrome: a systematic review and network meta-analysis

Abstract Background Metabolic syndrome (MetS) is a major risk factor for type 2 diabetes mellitus and cardiovascular disease. This systematic review and network meta-analysis compared the effects of different exercise regimens on metabolic outcomes in adults with MetS. Methods Ra...

Safflower yellow investigates AMPK/mTOR-mediated autophagy to mitigate sevoflurane-induced neuroinflammation and cognitive dysfunction

Background Perioperative neurocognitive disorders, including postoperative cognitive dysfunction (POCD), remain clinically important after surgery and anesthesia. This study evaluated the prophylactic neuroprotective effects of safflower yellow (SY) in a rat model of sevoflurane-...

PET imaging enables direct evaluation of ferroptosis in HCC synergistically induced by anti-PD-L1 and Olaparib

Abstract The efficacy of PET image-guided immune checkpoint inhibitor-based therapy in hepatocellular carcinoma (HCC) management is limited by the lack of direct monitoring of therapeutic response. A key challenge is the mismatch between the therapeutic pathway and the imaging ta...

The Dual-Ribozyme RNA Ladder and RNA-Calibrated ssDNA Marker System for Inexpensive RNA Size Standards

Abstract RNA size markers are essential for denaturing Urea-PAGE analysis, but commercial RNA ladders are expensive, available only in fixed size ranges, and require careful handling to prevent RNA degradation. Here, we developed a modular dual-ribozyme platform for preparing RNA...

Combined metabolomics and network pharmacology explore the protective mechanism of bornyl acetate against cognitive impairment in chronic sleep-deprived mice

Abstract Chronic sleep deprivation (CSD) is currently a serious global public health concern, and CSD induces numerous diseases, including cognitive impairment. Bornyl acetate (BA) possesses a brilliant anti-inflammatory activity and plays a favorable role in neuroinflammation. T...

Community-based traditional Chinese medicine health service use among older adults and provider-reported delivery barriers in Wuhan, China: an explanatory sequential mixed-methods study

Population ageing and multimorbidity are increasing the need for accessible community-based care, while service use may vary according to service availability, affordability, and delivery capacity. In China, community-based traditional Chinese medicine (TCM) health services repre...

Breviscapine attenuates myocardial ischemia-reperfusion injury by inhibiting ferroptosis via activation of NRF2/SLC7A11/GPX4 pathway

To explore the protective effect of Breviscapine against myocardial ischemia-reperfusion (I/R) injury and its mechanism. The in vivo and in vitro models of myocardial I/R injury were first established and treated with different concentrations of Bre. The myocardial infarct size w...

Association between PSQI and composite endpoints in ALS patients: a retrospective cohort study from China

Abstract Background Sleep disturbance is common in patients with amyotrophic lateral sclerosis (ALS), but its association with severe clinical outcomes remains insufficiently characterized. We aimed to investigate the association between baseline sleep quality, assessed using the...

Clockophagy in Alzheimer’s Disease: An Emerging Hypothesis Linking Circadian Dysfunction to Ferroptotic Vulnerability

Alzheimer’s disease (AD) is frequently associated with sleep disturbances and circadian dysfunction, which may precede or accompany clinical manifestations and have been associated with amyloid-β (Aβ) pathology, tau abnormalities, oxidative stress, lipid peroxidation, and neurona...

Factors influencing healthcare providers’ adoption of artificial intelligence-based clinical decision-support systems: a systematic review using the CFIR framework

Artificial intelligence-based clinical decision-support systems (AI–CDSS) are increasingly used in healthcare, but their adoption by healthcare providers (HCPs) remains challenging. Reported determinants span technological, organizational, individual, and implementation-related l...

Cadmium distribution and tolerance mechanisms in Artemisia argyi under cadmium stress

The dried leaf is the medicinal organ of Artemisia argyi, but the relationships among Cd allocation, physiological defense, cellular injury, and transcriptional responses remain incompletely resolved. We evaluated these responses after 14 d of hydroponic exposure to 0–40 mg/L Cd....

Allelochemicals of Atractylodes lancea: Toxicity, plant response signaling, and natural fallow degradation

Allelochemicals, released by crop roots, frequently cause pathogen invasion and suffer losses due to their potential hazards to plant growth and soil microenvironment. Natural fallow received prominent attention in tackling agroecological problems and soil bioremediation. In this...

Mortality trends and geographic disparities in motor neuron disease among US adults aged 25 years and older, 1999–2024: a serial cross-sectional study using CDC WONDER

To examine long-term trends in mortality from motor neuron disease (MND) among adults aged 25 years and older in the United States from 1999 to 2024 using the CDC WONDER database, and to assess differences by demographic characteristics, geographic region, and urbanization level....

Comparative metabolomic and microbiome characterization of herbal and traditional Baijiu during in vitro gastrointestinal digestion

BACKGROUND: Herbal Baijiu (HB) and traditional Baijiu (TB) contain complex chemical matrices whose metabolic fate during gastrointestinal digestion and subsequent impact on gut microbiota remain unclear. This study systematically compared HB and TB through in vitro gastrointestin...

Development and preliminary psychometric evaluation of an oral health questionnaire for chronic kidney disease (CKD-OHQ)

Oral health problems are common among patients with chronic kidney disease. Chronic kidney disease is associated with a broad range of oral manifestations, while poor oral health may accelerate chronic kidney disease progression, increase the risk of complications, and contribute...

Programming Hydrogel Release Kinetics to Tissue Healing Phases: From Network Design to Therapeutic Synchronization

The sequential phases of tissue healing—inflammation, proliferation, and remodeling—demand distinct pharmacokinetic profiles that conventional drug delivery systems fail to provide, creating a “chronotherapy gap” that contributes to chronic wound pathologies. Hydrogels, with thei...

Drosophila melanogaster Models for Natural Product Discovery: Cross-Disease Conserved Signaling Networks and a Generalizable Translational Pipeline

Drosophila melanogaster shares approximately 75% of human disease-related genes and possesses sophisticated genetic toolkits, including GAL4/UAS, CRISPR-Cas9, and RNA interference (RNAi), making it a rapid, cost-effective, and genetically tractable in vivo platform for natural pr...

Complete biosynthesis of the clinical agent escin Ia

Abstract Escin Ia is a barrigenol-type triterpenoid specific to Aesculus seeds and is widely utilized in clinical practice for the treatment of cerebral edema, swelling, and venous insufficiency. Here, we fully elucidated its biosynthetic pathway by identifying ten previously unc...

Structural insights into a substrate translocation pathway revealed by the RND efflux pump complex MexJK from Pseudomonas aeruginosa

Resistance–nodulation–cell division (RND) efflux pumps are the major cause of multidrug resistance in Pseudomonas aeruginosa . The inner membrane protein MexK from P. aeruginosa is a narrow-spectrum RND transporter. It assembles with membrane fusion protein MexJ and outer membran...