Hangzhou Academy of Agricultural Sciences

CRISPR/Cas12a-Based Aptasensor for Cas9 Protein Detection in Spiked Food Matrices

Residual gene editors are a concern associated with the widespread application of CRISPR/Cas9 technology. Here, we developed a CRISPR/Cas12a-based fluorescent aptasensor for the qualitative and quantitative detection of Cas9 protein. Secondary structure prediction and molecular d...

Branched-type nanocluster-junction fluorofluidic-gels for interface-adaptive thermal conduction and mechanical resistance

With the miniaturization and high integration of microelectronic devices, efficient thermal management is increasingly critical. Conventional thermal interface materials often suffer from a certain degree of mismatch between their thermal conductivity and contact thermal resistan...

Beyond In Vitro Brightness: A Matched-Probe Study of Chemiluminescence Contrast for In Vivo Peroxynitrite Imaging

Probe selection for in vivo chemiluminescence imaging commonly relies on intensity and tissue-overlay measurements performed in vitro, yet these measurements do not reproduce the activation background, probe distribution, and optical environment of a living animal. Here, we test...

The CED paper

We present the Category Error Detector (CED), a diagnostic instrument for identifying structural misalignment in any system defined by a fundamental tension. The CED operates on five nodes—Axes, Interface, Projection, Lagrangian, Attractors—and returns a binary score for each, yi...

Lotus (Nelumbo nucifera)-based co-culture systems reshape gut microbiota and enhance health and microbial stability in Chinese soft-shelled turtle (Pelodiscus sinensis)

Abstract Background The Chinese soft-shelled turtle ( Pelodiscus sinensis ) is a valuable aquaculture species in East/Southeast Asia; however, intensive farming has caused pathogen prevalence and antibiotic overuse, threatening sustainability. The gut microbiome plays a critical...

Higgs-Stiffness-Gravity (revised)

We present a revised formalization of Higgs-Stiffness Gravity, a unified framework in which the dark sector arises from the tensorial elastic response of the Higgs condensate to spacetime curvature. The model introduces one genuinely new free parameter (ξ), alongside standard cos...

The layers of time (revised)

We present the final revised geometry of time derived from the Meta- physic of the Intelligible. Time is not a single dimension but a cascade of projections from the ground to the contingent world. Four layers are iden- tified: Super Lagrangian timelessness, cosmic time (entropy...

The axiomatic interface

Axioms are not arbitrary conventions — they are projections of the Logic of Identity, the axiomatic source from which all formal systems emerge. This paper explores how formal systems touch the ground through axioms, how they remain open, and how they are recognized as coherent p...

A review of the metaphysic of the intelligible

This is the final paper of the corpus — a synopsis, a retro view, and a release. We present the Metaphysic of the Intelligible as a neutral monism and a structural rationalism: a geometry that does not claim to describe reality as it is, but offers a lens for engaging with realit...

CED as a python module for coherence in complex computation

We present a validated Python implementation of the Category Error Detector (CED) as a portable, self-contained diagnostic module. The CED scans any system—AI, simulation, or theoretical model—for structural misalignment across five nodes: Axes, Interface, Projection, Lagrangian,...

CED- two posthumanisms

We apply the Category Error Detector (CED) to two distinct instantiations of posthumanism: one grounded in thestructural ethics of invariant worth, and one coupled to game theory’s local attractor of strategic predominance. We show that posthumanism, in its moderate form, is a co...

Mathematics as discovery: geometry of the intelligible

We present a geometric framework for understanding why mathematics feels like discovery, not invention. Drawing on the Metaphysic of the Intelligible, we show that mathematical creation is a projection from the ground through the Identity Tensor, guided by tensorial rules and dri...

Metabolomics-Based Analysis of Quality Differences in Dendrobium officinale Leaves Under Different Flower Treatments

Abstract To elucidate how flower retention regulates leaf quality formation in Dendrobium officinale and to provide a theoretical basis for high-quality leaf cultivation and high-value utilization of byproducts, this study used D. officinale leaves as an experimental material and...

In Situ Tracking of Membrane HER2- Antibody–Drug Conjugate Interaction with Surface PlasmonResonance Imaging

Abstract Antibody–drug conjugates (ADCs) rely on specific recognition of tumor-associated membrane receptors to achieve targeted intracellular drug delivery, yet in situ characterization of their interaction dynamics remains limited. Here, we develop a single-cell plasmonic imagi...

Metal‐Based Combination Therapies in Cancer: Mechanistic Principles and Emerging Opportunities

Metal-based anticancer agents occupy a distinctive niche in cancer therapy owing to their exceptional coordination versatility, redox activity, and tunable photochemical properties, which enable simultaneous engagement of multiple cellular pathways. Despite the clinical success o...