Hamilton Health Sciences

Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells

We present a protocol for isolating and culturing murine adipose progenitor cells (APCs) from adipose tissue. We describe steps for tissue dissection, unit-normalized enzymatic digestion, stromal vascular fraction (SVF) isolation, APC enrichment, and cell culture. We then detail...

Ultraprocessed Food Intake and Adiposity, Vascular Brain Injury, and Cognition in Middle-Aged Adults

Importance Ultraprocessed foods (UPFs) account for a substantial and increasing proportion of dietary intake in Canada. Although UPFs are consistently associated with adiposity and cardiometabolic disease, their associations with subclinical vascular brain injury (VBI) and cognit...

Diagnoses associated with reimbursement of cannabis for medical purposes among Canadian Veterans, 2016-23

Introduction : Canadian Veterans who are authorized to access cannabis for medical purposes (CMP) by a health care practitioner may be reimbursed for the cost by Veterans Affairs Canada (VAC). To assess eligibility for reimbursement, VAC receives and reviews medical authorization...

Intersectionality in HIV-related policy documents affecting people who use drugs in Ontario, Canada: a policy document analysis

In 2023 Canadian surveillance data, injection drug use accounted for 13.6% of new HIV diagnoses among males and 28.4% among females in exposure-category data. This study identified the presence of intersectionality language among health policies that affect access to HIV related...

Use of Dual Orexin Receptor Antagonists for Insomnia and the Risk of Falls and Fractures: A Systematic Review and Meta‐Analysis

ABSTRACT Background Falls are the leading cause of injury in older adults. Sedative‐hypnotic use is a known risk factor, but their use for insomnia continues to be common. Dual orexin receptor antagonists (DORAs) are a new, potentially safer class of medications for insomnia. We...

Semi-recumbent position for the prevention of ventilator-associated pneumonia in adults requiring mechanical ventilation

RATIONALE: Ventilator-associated pneumonia (VAP) is associated with increased mortality, hospital stay, and healthcare costs in critically ill patients. Although guidelines recommend a semi-recumbent position for VAP prevention, the evidence remains uncertain due to limitations i...

Engaging youth in end-of-life care: The 3 Wishes Project experience

Background Citizen engagement can create a community of compassion for dying hospitalized patients. Objectives To describe a family co-designed end-of-life intervention engaging schoolchildren, and characterize dying hospitalized patients and their terminal wishes. Methods This c...

Amniotic fluid index versus maximum vertical pocket for predicting adverse perinatal outcomes in singleton term pregnancies: A retrospective cohort study

OBJECTIVE: Amniotic fluid assessment is central to antepartum fetal surveillance. Current guidelines favor the maximum vertical pocket (MVP) over the amniotic fluid index (AFI), as AFI increases oligohydramnios diagnosis and labor induction rates without improving perinatal outco...

From Awareness to Action: Co‐Designing Culturally Safe and Equitable Rehabilitation in Canada – Protocol for the Two‐Phase Ireach Qualitative Study

ABSTRACT Background Inequities in access, engagement, and outcomes persist across outpatient rehabilitation services in Canada, disproportionately affecting Indigenous Peoples, newcomers and refugees, gender‐ and sexually‐diverse individuals, and people with lower income. Althoug...

Identifying methodological guidance used for systematic reviews, clinical practice guidelines, and health technology assessment for rare diseases: a scoping review protocol.

OBJECTIVE: This scoping review will identify methodological guidance relevant to systematic reviews (SRs), clinical practice guidelines (CPGs), and health technology assessments (HTAs) for rare diseases, and examine methodological challenges associated with their development. INT...

Cross-sectional evaluation of anthropometric measures vs MRI-measured visceral and hepatic fat in a Canadian cohort

Abstract Context Accurate assessment of excess body fat and its cardiometabolic risk is essential in clinical and epidemiologic research. Body mass index (BMI), although widely used, does not capture visceral or ectopic fat. Objective To evaluate how anthropometric measures—BMI,...

Milvexian with Antiplatelet Therapy after Acute Coronary Syndrome Event

BackgroundPatients are at increased risk for recurrent ischemic events after an acute coronary syndrome event. Milvexian, an oral factor XIa inhibitor, may reduce the risk of major adverse clinical events with minimal bleeding risk. MethodsIn this phase 3, randomized, placebo-con...

Congenital Tuberculosis in a 2-Week-Old Neonate With an Asymptomatic Mother

Congenital tuberculosis (TB) is a rare condition, and diagnosis is challenging due to its nonspecific clinical presentation and rarity. It has a very high case-fatality rate (estimated at 30%–50%). We report the case of a 2-week-old male neonate, born from an uncomplicated diamni...

Statistical methods, outcome structures, and research designs in hybrid type II effectiveness-implementation studies: protocol for a scoping review

<ns5:p>Background Hybrid type II effectiveness-implementation studies evaluate health intervention and implementation strategy effectiveness simultaneously. While the emphasis on each “side” varies within hybrid type II studies, the focus of this review is those that attempt a mo...

Interpersonal Predictors of Youth Internalizing Symptom Trajectories During the COVID‐19 Pandemic

INTRODUCTION: The COVID-19 pandemic disrupted youths' interpersonal relationships, posing risks for internalizing distress. Yet, evidence remains limited beyond the early pandemic waves and with changing interpersonal contexts. This study examined trajectories of youth internaliz...