G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms

Mycobiota of the Bottom Sediments of the White Sea: Metabarcoding and Cultural Method

Abstract Knowledge of the composition of fungi in sediments is necessary to understand many biological processes, the decomposition of organic substances in aquatic ecosystems in particular. However, the diversity of fungi in the Arctic seas remains poorly studied, and single stu...

Preservation of Strictly Acidophilic Autotrophic Bacteria Acidithiobacillus ferrooxidans by Lyophilization Using Steel

Abstract Acidithiobacillus ferrooxidans are strictly acidophilic bacteria, which are used in metal recovery practices worldwide. Guaranteed preservation of the viability and genetic potential of A. ferrooxidans strains remains important due to the need for long-term maintenance o...

Reassessment of IRES Element Activity in Komagataella phaffii Using a Standardized Bicistronic System: Cryptic Promoter Activity Mimics Cap-Independent Translation

Internal ribosome entry site (IRES) elements have been proposed for constructing polycistronic mRNAs in Komagataella phaffii, but their reported activity is inconsistent and has not been evaluated under standardized conditions. Here, a standardized bicistronic EGFP-IRES-mKate2 sy...

The Effect of Mutations in Glycanase Genes on the Formation of Exo-oligosacharides in Nodule Bacteria Rhizobium leguminosarum bv. viciae VF39

Abstract In order to identify the mechanisms underlying the formation of low-molecular-weight forms of acidic exopolysaccharide in the symbiotic bacteria Rhizobium leguminosarum bv. viciae, a comparative analysis of exo-oligosaccharide production was conducted in the wild-type st...