China Light Industry Press (China)

Multifaceted Cross-Rational Modification of the α2,3-Sialyltransferase MsST for High-Efficiency Production of 3′-Sialyl-3-fucosyllactose via One-Pot Cascade Reaction

Abstract 3′-Sialyl-3-fucosyllactose (3′S3FL) is a representative fucose-containing sialylated human milk oligosaccharide with distinctive biological functions, but its in vitro synthesis is limited by the poor tolerance of α2,3-sialyltransferases toward fucosylated acceptors. Her...