Chan Zuckerberg Biohub San Francisco

Prehistoric global migration of vanishing gut microbes with humans

The gut microbiome is crucial for health and is affected strongly by lifestyle1. Many microorganisms commonly found in non-industrialized populations are disappearing or have become extinct in industrialized populations2–6. Studying which microorganisms have been long-term reside...

PrimerDesigner: Designing Efficient Primers for Synthesizing Large Protein Libraries Without Cross-Hybridization

Efficient synthesis of large protein libraries is key to making high-throughput biochemistry scalable. Advances in DNA microarray technology now allow more than 170,000 designed oligonucleotides to be synthesized in a single array, dramatically reducing cost and enabling pooled a...

Effects of Chemical Fixation on Stimulated Raman Scattering Microscopy

Abstract Stimulated Raman scattering (SRS) microscopy enables label-free imaging of cells and tissues in their native state with chemical specificity. However, there are often experimental advantages of chemical fixation of samples prior to imaging, which can introduce perturbati...

Benchmarking three simple DNA staining-based image metrics for live-cell tracking of chromatin organization

Quantifying chromatin-state dynamics in living cells remains challenging, in part because most methods require fixation or cell lysis. Here, we introduce two simple DNA image-derived metrics - Diffuse Signal Index (DSI)and 1-Gini - and benchmark them against the coefficient of va...

Failed metabolic adaptation to stress contributes to epidermal cell adhesion defects in Darier disease

Congenital pathogenic gene variants may not elicit symptoms until later in life, highlighting the importance of identifying extra-genetic factors influencing the onset and severity of heritable diseases. We explored this in Darier disease (DD or ATP2A2 -nEDD), an autosomal domina...