Centre for Structural Systems Biology

Global analysis of thermal and chemical denaturation using CheMelt : Thermodynamic dissection of highly thermostable de novo designed proteins

ABSTRACT De novo protein design often produces thermostable proteins that denature above 100 °C, which complicates the analysis of their stability. Thermostable proteins can be unfolded by combined chemical and thermal denaturation followed by global analysis of multiple melting...

Clathrin adaptors drive phase separation in endocytosis and trafficking

Abstract Liquid–liquid phase separation (LLPS) underlies the formation of biomolecular condensates that organize processes, including endocytosis and membrane trafficking. Although LLPS has been implicated in clathrin-mediated pathways, the contribution of adaptor proteins to con...

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF ‐Tu for AMPylation

Fic enzymes mediate diverse post-translational modifications, including adenosine monophosphate (AMP) transfer and removal, referred to as AMPylation and deAMPylation, respectively. We identified the prokaryotic translation elongation factor Tu (EF-Tu) as an AMPylation target of...

The membrane-to-cortex distance regulates mDia1 activity to control cortical mechanics

Abstract The shape of animal cells is controlled by their surface, which comprises the cell cortex, a peripheral actin network, tethered to the plasma membrane by membrane-to-cortex attachment proteins. Changes in cortical components have long been considered to dominate the regu...