Center for Excellence in Molecular Cell Science

UBE2K-FBXO11 complex activates Wnt signaling by stabilizing β-catenin through lysine 63-linked polyubiquitination

Abstract Β-catenin is regulated by phosphorylation and lysine (K)48-linked ubiquitination, which target it for proteasomal degradation. While the degradation machinery of β-catenin, including the destruction complex, has been extensively studied, regulators that stabilize β-caten...

Cotranslational membrane protein biogenesis by an EMC-bound translocon

Secretory and membrane protein biogenesis occurs at ribosome-bound translocons in the endoplasmic reticulum (ER) membrane. Translocons consist of the Sec61 protein-conducting channel and various maturation factors including chaperones, modification enzymes and membrane insertases...

Mitotic assembly of respiratory supercomplexes ensures accurate cell division and fuels tumor growth

As cells progress from interphase into mitosis, fluctuating metabolic demands coincide with mitochondrial fission. However, the mechanisms by which mitochondria coordinate morphological changes and metabolic adjustments during mitosis remain poorly understood. Using proteomic ana...

Mechanistic Basis for 5-HT2AR Over 5-HT2BR Activation

Selective 5-HT2AR agonism without 5-HT2BR activation is critical for safe psychedelic-inspired therapeutics. Here, we systematically probe the ligand-binding pockets of 5-HT2AR and 5-HT2BR and identify steric and conformational constraints at the side-extended pocket (SEP) and ex...

Asymmetric RBBP7 regulates the first cell fate decision of early mammalian embryos

Asymmetric transcription of noncoding RNA LincGET is currently recognized as the earliest event regulating the first cell fate decision in mammalian embryogenesis. However, whether key protein factors modulate this process remains elusive. Here, we identify RBBP7 as the earliest...