Center for Biologics Evaluation and Research

An Explanation forthe 32 and 54 Da Losses Seen in N -Linked GlycanPermethylation Reactions

Abstract Permethylation is a widely used derivatization technique for mass spectrometry-based glycan analysis, applicable to both research and drug product characterization. It stabilizes sialic acids, improves glycan ionization, and is commonly used to analyze PNGase F-released...