Bioscience Research

Single-Tube Six-Channel Ultrafast PCR with Melting-Curve Discrimination for Seven Respiratory Pathogens

Abstract Expanding single-tube multiplexing beyond the number of fluorescence channels on a real-time PCR instrument is a persistent challenge for point-of-care testing (POCT). Here, we break this barrier by leveraging melting temperature (Tm) as an extra information dimension. B...