Amsterdam UMC Location University of Amsterdam

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synovium

Abstract Real-time quantitative PCR (RT-qPCR) is a widely used method for gene expression analysis; however, its accuracy depends on the selection of stable reference genes for normalization. To date, no study has systematically identified suitable reference genes for primary hum...

Schizophrenia research in 2026: report from the annual meeting of the Schizophrenia International Research Society

Abstract SIRS 2026, held under the theme “Schizophrenia Research in the Era of Artificial Intelligence,” brought together researchers, clinicians, and persons with lived experience to take stock of a field whose tools for measuring, predicting, and intervening in psychosis have e...

Temporal Dynamics of Complement Activation and Outcome in Patients With Acute Ischemic Stroke

BACKGROUND: Immune-mediated inflammatory responses exacerbate brain injury and affect the prognosis in acute ischemic stroke. METHODS: We characterized longitudinal complement activation after acute ischemic stroke using serial plasma samples from 10 patients and 8 controls. Init...

Post-acute sequelae of COVID-19 (PASC) are associated with distinct clinical phenotypes two years after SARS-CoV-2 infection: A data-driven approach

It is important to determine whether the persistent cognitive and fatigue complaints, as part of post-acute sequelae of COVID-19 (PASC), are associated with underlying patterns and specific symptoms clusters. Therefore, the aim of the present study was to investigate symptom clus...

The Influence of Deep Hypothermic Circulatory Arrest on the Incidence of Postoperative Acute Kidney Injury After Pulmonary Endarterectomy

Background: Pulmonary endarterectomy (PEA) in patients with chronic thromboembolic pulmonary hypertension (CTEPH) is performed using phases of deep hypothermic circulatory arrest (DHCA). DHCA has been discussed as a risk factor for cardiac surgery-associated acute kidney injury (...

Exploring transcriptomic and genomic latent variable correction approaches in differential expression analysis

Abstract Background Differential expression analysis is a central tool for studying the biological processes altered in human diseases via transcriptomic signatures. However, transcriptomic datasets are systematically confounded by latent variables from two distinct sources: unme...

Ultrasound protocols used to detect vascular gas emboli in divers: a systematic review

Introduction: Venous gas emboli (VGE) detected via ultrasound can be used as a surrogate marker for decompression stress. While Doppler ultrasound is the historical gold standard, two-dimensional (2D) ultrasonography offers advantages for on-site monitoring, including a wider fie...

Impact of neoprene wetsuits on lung volumes and work of breathing: implications for military diver safety and performance

Introduction: Neoprene wetsuits may impose mechanical constraints on the chest wall, potentially altering respiratory function. This study investigated the impact of neoprene wetsuits on lung volumes, airway mechanics, and work of breathing (WOB) in healthy male divers. Methods:...

Quality standards for preclinical research in cardio-oncology

Preclinical research in cancer therapy-related cardiovascular toxicity (CTR-CVT) is a major domain of cardio-oncology and has provided biological explanation for the cardiovascular adverse effects of many cancer therapies. However, the choice of the experimental models to investi...

The identification of a PRKACA duplication at 19p13.12 in a female with PPNAD and thyroid carcinoma after a 20-year diagnostic journey

Abstract Rare pathogenic variants affecting components of the evolutionary conserved cAMP/protein kinase A (PKA) signalling pathway are implicated in a spectrum of adrenocortical disorders. Germline inactivating PRKAR1A variants leading to constitutive PKA activation, underlie pr...

Data-Driven Fatigue Trajectories in Multiple Sclerosis and Their Predictors

Fatigue is one of the most disabling symptoms of multiple sclerosis (MS), yet its longitudinal trajectories and determinants remain poorly characterized. We aimed to identify trajectories of cognitive and physical fatigue in relapsing-remitting MS (RRMS) and to determine baseline...

Converging pathomechanisms link SARS-CoV-2 infection with major neurodegenerative diseases

Neurological complications are among typical clinical manifestations, both during the acute phase of SARS-CoV-2 infection and long-term post-acute COVID-19 sequelae, i.e., Long COVID. A growing body of evidence connects SARS-CoV-2 infection to systemic inflammation, peripheral im...