Amsterdam institute for Immunology and Infectious Diseases

Identification of stable reference genes for quantitative real-time PCR in human fibroblasts from lymph nodes and synovium

Abstract Real-time quantitative PCR (RT-qPCR) is a widely used method for gene expression analysis; however, its accuracy depends on the selection of stable reference genes for normalization. To date, no study has systematically identified suitable reference genes for primary hum...

Sex work or male-with-male sexual activity and the risks of HIV and hepatitis C acquisition among people who inject drugs: a global systematic review and meta-analysis

Background People who inject drugs (PWID) may engage in sexual behaviours that increase their HIV and hepatitis C virus (HCV) risk, including sex work and male-with-male sexual activity. We undertook a global systematic review to assess whether PWID who engage in these behaviours...

Liver X receptor (LXR) signaling in macrophage inflammation and cardiometabolic disease.

Cardiometabolic diseases profoundly remodel tissue immune landscapes, characterized by the accumulation of diverse macrophage subtypes. Among these, lipid-associated macrophages (LAMs) have emerged as an important subset that is frequently observed in cardiometabolically affected...