Allen Institute

A DNA Typewriter records the cell lineage history of a mouse, from zygote to late organogenesis

Mammalian biology unfolds over time, within tissues and organs opaque to our eyes and instruments. We applied DNA Typewriter, a sequential molecular recorder, to record the cell lineage of a mouse over nearly two weeks of development. From one embryo, we reconstruct a time-calibr...

Perioperative pelvic floor physical therapy in endometriosis surgery: analysis workbook and code for a systematic review and meta-analysis

Supplementary materials for the systematic review and meta-analysis Perioperative Pelvic Floor Physical Therapy in Endometriosis Surgery: A Systematic Review and Meta-analysis. Changes in this version. This version supersedes version 1.0.0 and corresponds to the submitted manuscr...

Neocortical long-range inhibition promotes cortical synchrony and sleep

Abstract Sleep and wakefulness are associated with distinct cortical patterns of rhythmic activity 1 . During low-arousal states such as slow-wave sleep, synchronous low-frequency rhythms dominate activity across widespread cortical regions. Although inhibitory neurons are increa...

Beyond benchmarking: an expert-guided consensus approach to spatially aware clustering

Spatial omics technologies have revolutionized the study of tissue architecture and cellular heterogeneity by integrating molecular profiles with spatial localization. In spatially resolved transcriptomics, delineating higher-order anatomical structures is critical for understand...