Ajinomoto (Japan)

Loop engineering and activity improvement of TEV protease by a phagemid-based selection system

Abactstr Loop engineering of enzymes remains challenging due to high flexibility and conformational complexity, posing a bottleneck for deep-learning-based design. Here, we constructed mutant libraries for three loops of TEV protease to assess combining directed evolution with de...

Leucine-Enriched Essential Amino Acids in Clinical Practice: A Narrative Review

Muscle wasting driven by aging, chronic disease, or intensive exercise undermines mobility, metabolic health, and survival—yet the people who lose the most muscle are often those least able to eat more protein. Leucine-enriched essential amino acid (LEAA) mixtures combine a leuci...

Modeling Canine Hemangiosarcoma Progression Using Patient-Derived 2.5D Organoids and Orthotopic Xenografts

Canine hemangiosarcoma (HSA) is a highly aggressive vascular malignancy encountered in veterinary practice and exhibits histopathological and molecular similarities to human angiosarcoma (AS). Despite its clinical importance, the molecular mechanisms driving canine HSA remain poo...