Academy of Military Medical Sciences

Route-Dependent Effects of Pre-Existing Anti-Ad5 Immunity on Systemic, Mucosal and Lymph Node B Cell Responses to an Ad5-Vectored Vaccine

Background/Objectives: Pre-existing anti-Ad5 immunity (PEI) impairs the immunogenicity of intramuscular (IM) Ad5-vectored vaccines. Whether respiratory immunization can better preserve vaccine-induced immunity remains unclear. Methods: We established a murine PEI model to compare...

Biosynthetic DNA origami nanostructures: harnessing E. coli-phage systems as nanofactories

DNA origami nanostructures (DONs) serve as versatile nanocarriers with promising application potential, but high production cost and low yield limit their development. The biosynthetic approach based on the Escherichia coli ( E. coli )-phage system offers an effective route for t...

CRISPR/Cas13a-Based Nucleic Acid-Functionalized Discrete Floating-Gate Field-Effect Transistor for Amplification-Free Rapid Nipah Virus Detection

Abstract Nipah virus (NiV) is a highly lethal zoonotic pathogen, and existing rapid diagnostic tools are scarce and reliant on nucleic acid amplification. In this study, we designed and fabricated a thin-film field-effect transistor with an IGZO channel and, for the first time, c...

Single-Cell BCR and Transcriptome Analysis Revealed Potent Neutralizing Antibodies in Response to HAdV-4 Infection

Human adenovirus type 4 (HAdV-4) is a predominant serotype causing acute respiratory infections, for which no antibody drugs have been clinically approved to date, resulting in an urgent unmet medical need. This study aimed to identify highly potent neutralizing antibodies by dis...

Multivalent antiviral conjugates enhance activity against Influenza via cross-linking viruses

Abstract Long-acting drug–Fc conjugate (CD388) has emerged as a new class of antivirals against influenza A and B, yet the mechanisms governing how Fc conjugation reshapes small-molecule function remain undefined. Here we establish a programmable multivalent antiviral conjugates...

ESAT-6 and CFP-10 in tuberculosis: bridging virulence to diagnostic applications

Tuberculosis (TB) remains a major global public health challenge. Accurate detection of Mycobacterium tuberculosis ( Mtb ) infection, particularly latent tuberculosis infection (LTBI), is essential for targeted preventive treatment and progress toward TB elimination. The traditio...

mRNA lipid nanoparticle vaccines: current status, challenges and future prospects

Abstract mRNA vaccines have become a clinically validated vaccine platform, as demonstrated by the success of COVID-19 vaccines such as Comirnaty and Spikevax, owing to their rapid design, manufacturing scalability, and capacity to induce in situ antigen expression. A key factor...

Methyl-donor inhibitors relax 3D genome structures to promote totipotent reprogramming

The 3D genome safeguards cell identity while shaping developmental plasticity, yet its coordination with metabolism during cell fate transitions remains unclear. Here, we use a mouse totipotent stem cell induction system to dissect the role of methyl-donor metabolism in 3D genome...

VIDA: A programmable gasdermin-mediated strategy to broaden mRNA therapeutics

Pyroptosis, a gasdermin (GSDM)-driven form of inflammatory programmed cell death, has long been recognized for its dual function: direct elimination of infected or transformed cells and activation of the immune system via released cellular contents and pro-inflammatory mediators....

An engineered AAV variant with high-efficiency outer retinal tropism for gene delivery

Retinal degenerative diseases, such as Stargardt disease and age-related macular degeneration (AMD), are leading causes of irreversible blindness, and AAV-based gene therapy has become a key therapeutic option. For most natural AAV serotypes, however, efficient transduction of th...

One-Pot Ketone Functionalization of EPDM toward Recyclable and Creep-Resistant Elastomers Cross-Linked with Biobased Amines

Abstract Introducing dynamic covalent networks into conventional ethylene-propylene-diene monomer (EPDM) rubber offers a promising strategy for developing thermally reprocessable elastomers; however, it requires the introduction of reactive functionalities onto the chemically ine...

Targeted Inhibition of Intracellular Coxiella burnetii by Overcoming the Vacuolar Barrier with Autologous Exosomal Doxycycline

Abstract Coxiella burnetii, the causative agent of Q fever, is an obligate intracellular bacterium that replicates within the Coxiella-containing vacuole (CCV), a lysosome-like compartment. The acidic microenvironment within the CCV impairs the activity of weak-base antibiotics s...

A Recombinant RABV-G-Based Chemiluminescence Immunoassay for Quantitative Detection of Rabies Virus Antibodies

Background: Rabies is a nearly 100% fatal zoonosis, and a rabies virus-neutralizing antibody (RVNA) titre of ≥0.5 IU/mL is the recognized correlate of protection, making post-vaccination serological monitoring essential. However, the gold-standard methods (RFFIT and FAVN) are tim...

Vitamin D ameliorates the inflammation, oxidative stress and mitophagy to enhance the protective effects of MSCs on the pancreatic injury

Recently, mesenchymal stem cells (MSCs) therapy has emerged as a revolutionary breakthrough in the treatment of T1DM. Nevertheless, its therapeutic efficacy is controversial due to the influence of diverse micro-environment including vitamin D (VD) deficiency. Moreover, it still...

Identification of differential topologically associating domains from low sequencing depth and pseudo-bulk chromatin contact maps

Topologically associating domains (TADs) are fundamental units of 3D genome architecture that shape gene regulation. Comparative analyses of TADs across biological conditions have revealed their involvement in development and disease. However, accurately identifying differential...

Dinuclear Platinum(II)Complexes Targeting DNA andHuman Serum Albumin: Electrostatic Binding and Apoptosis-Driven AntiproliferativeActivity

Abstract Two novel dinuclear platinum(II) complexes, [{Pt(1,3-pnd)Cl}2(μ-4,4′-bipy)]Cl2 (Pt1) and [{Pt(1,3-pnd)Cl}2(μ-4,4′–SS–bipy)]Cl2 (Pt2), were synthesized and characterized by elemental analysis, UV–vis, and 1H/13C NMR spectroscopy. Spectroscopic and electrochemical studies...

Comprehensive genomic characterization of extraintestinal pathogenic Escherichia coli isolated from neonates: multiple center insights into virulence, resistance, and transmission dynamics

Neonatal extraintestinal pathogenic Escherichia coli (ExPEC), which can cause severe long-term sequelae by systemic infections, is gradually becoming the primary pathogen threatening neonatal health. The lack of large-scale genomic epidemiological investigation hinders further un...

Hydrogen Sulfide-Regulated NF-κB Signaling via Persulfidation: A Review

Hydrogen sulfide (H2S) is an important endogenous gaseous signaling molecule that regulates diverse physiological and pathological processes through protein persulfidation. As a central regulator of inflammation and immune responses, NF-κB signaling is precisely controlled by pos...

MINK1 inhibition suppresses triple-negative breast cancer by abrogating ALDOA S39 phosphorylation and reprogramming glucose metabolism

Triple negative breast cancer (TNBC) represents the most malignant subtype of breast cancer, with heightened invasiveness, frequent recurrence and poor prognosis. Deciphering the molecular mechanisms underlying TNBC pathogenesis and drug resistance is critical for developing prec...