A N Bach Institute of Biochemistry

The I130V and S229F Tropomyosin Mutations Associated With Congenital Heart Disease Disrupt Actin‐Myosin Interactions in the Atria

ABSTRACT Tropomyosin is a highly conserved α‐helical coiled‐coil protein that is involved in maintaining the structural integrity of the sarcomere and regulating contraction of striated muscle. Mutations in the TPM1 gene encoding cardiac α‐tropomyosin (Tpm1.1) are associated with...

Cryo-EM structure of ex vivo Sup35 yeast prion and the factors defining prion phenotype

Abstract Human prions and amyloids exhibit structural diversity that correlates with different pathological manifestations. Yeast prions represent a convenient model for studying fundamental properties of prions and their strain diversity. The Sup35 yeast prion has multiple struc...

Reassessment of IRES Element Activity in Komagataella phaffii Using a Standardized Bicistronic System: Cryptic Promoter Activity Mimics Cap-Independent Translation

Internal ribosome entry site (IRES) elements have been proposed for constructing polycistronic mRNAs in Komagataella phaffii, but their reported activity is inconsistent and has not been evaluated under standardized conditions. Here, a standardized bicistronic EGFP-IRES-mKate2 sy...