Zhenghong Xu

Metabolic engineering and deep learning-driven protein engineering for N-Acetylneuraminic acid biosynthesis in Escherichia coli

N-Acetylneuraminic acid (NeuAc) is a sialic acid valued in pharmaceuticals and infant nutrition, and microbial synthesis offers a scalable route once the pathway’s catalytic bottlenecks are relieved. We combine deep learning with metabolic engineering to build a high-titer NeuAc-...