Yunting Liu

Electroenzymatic Cascade for Chiral Hydroxylation of C–H Bond Using In Situ H2O2 Generation

Abstract Unspecific peroxygenase (UPO) catalyzes selective oxyfunctionalization of C–H bonds using H2O2 but suffers from enzyme instability and substrate solubility issues. To address these challenges, a cobalt and nitrogen co-doped carbon nanotubes electrocatalyst with two-elect...

Structure-Guided Engineering of Amine Dehydrogenase for Chiral Bulky β -Aryl Amine Synthesis: Dynamic Loop-to-Helix Shift and Hydrogen-Bond Network Reconstruction

Abstract Chiral β-aryl amines are critical structural motifs in various bioactive compounds and pharmaceuticals. Amine dehydrogenases (AmDHs) offer a promising sustainable approach to catalyze the asymmetric reductive amination of β-aryl ketones to chiral β-aryl amines. However,...

Disclosing Structural Determinants Governing Stereopreferences of Ketoreductases toward Reduction of Halogenated Aryl Ketones

Abstract Ketoreductases (KREDs) are widely used for asymmetric ketone reduction, yet predictable control of stereoselectivity remains challenging due to the limited understanding of structure–selectivity relationships. Here, we integrate site-directed mutagenesis and molecular dy...