Yanru An

Recombinant Collagenase-Assisted Production of Bovine Bone-Derived Peptides and EGFR-Guided Discovery of Osteogenic Oligopeptides

Abstract Bovine bone is a collagen-rich byproduct with potential for producing osteogenic peptides. A recombinant collagenase was heterologously expressed in Escherichia coliBL21(DE3), purified, and characterized. The enzyme showed a specific activity of 231.05 U/mg and hydrolyze...