Xialing Zhao

Visual and rapid detection of Fasciola spp. in fecal samples using a one-pot RPA-CRISPR/Cas12a assay

Fasciolosis caused by Fasciola spp. has substantial veterinary and public-health importance, while conventional fecal microscopy has limited sensitivity and PCR depends on laboratory equipment. This study aimed to develop a rapid, visual one-pot recombinase polymerase amplificati...