Wojciech P. Pawlowski

Immunolocalization of Meiotic Proteins in Maize

Meiosis is an important generator of genetic diversity in plants. Maize, with its large and conspicuous chromosomes, is an attractive model for cytological studies of meiosis. Immunolocalization employs the antigen–antibody interaction to visualize proteins within cells and tissu...

Detecting Meiotic Crossing-Overs in Maize Using Chromatin Immunoprecipitation-Sequencing (ChIP-seq)

During meiosis, homologous chromosomes engage in reciprocal exchanges of segments in a process known as crossing over (CO). About 85% of CO events in maize are products of the class I pathway. Class I COs are interference-sensitive, meaning that the formation of one CO reduces th...

Site-Specific Measurement of Meiotic Crossing-Over Rate with Droplet Digital PCR

Understanding the frequency and distribution of meiotic crossovers (COs) is critical for both fundamental studies on meiosis and for practical applications in plant breeding, where controlling recombination can accelerate crop improvement. Determining CO rates at specific genomic...