V R Kirubakaran

A Simple Modified Method for Long-Term Glycerol-Based Cryopreservation of Leptospira Spp. in Liquid Nitrogen

Introduction: A simple and reliable method for long-term preservation of Leptospira spp. is essential for maintaining reference strains and field isolates for diagnostic and research purposes. Although 10% glycerol is commonly used as a cryoprotectant for Leptospira preservation,...