Thanh Thi Ngoc Nguyen

Development and validation of a tail-extension PCR protocol for improved sanger sequencing of a short miR-16-5p-derived amplicon

Abstract Background MicroRNA-16-5p (miR-16-5p) plays an important role in gene regulation and has been implicated in several human diseases. Improved sequence verification of short miRNA-derived amplicons is important in assay development and control validation. Because stem-loop...