Tammy Kang

Investigation of the pBR322 Exclusion Effect using Putative Rop Mutant pBR322 Plasmid pCAWK

Rop, repressor of primer, protein has been implicated in causing or contributing to plasmid pBR322 exclusion from Escherichia coli host cells. A recent study successfully created pCAWK, a putative mutated pBR322 plasmid with a non-functioning Rop protein. Whole cell PCR was perfo...