Taeseong Woo

Tunable TIRF Microscopy: Simultaneous Visualization of Intracellular Dynamics at Multiple Depths

ABSTRACT We propose and demonstrate tunable total internal reflection fluorescence (TIRF) microscopy, a new method to achieve dynamic customized depth‐selective fluorescence excitation across the field of view. By simply adding a digital micromirror device (DMD) and a spatial fil...