Songkuan Zhuang

Exploiting Asymmetric Mismatch Sensitivity of Cas13a for Interference-Tolerant Single-CpG Methylation Analysis

Abstract Accurate single-CpG methylation analysis in dense regions is critical for cancer diagnostics but challenged by adjacent CpG interference. Here, we developed EPICATCH, an interference-tolerant ratiometric Cas13a biosensor to overcome this limitation. Exploiting LbuCas13a′...